Nuclear factor kappa B (NFkB) was identified as a sequence specific transcriptional activator that binds to the intronic enhancer of kappa light chain gene in B lymphocytes. NFkB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA). Both of these subunits exhibit sequence homology to the protooncogene c-Rel. The p50 has an isoform called p49/p52,and both proteins are derived from the amino-terminal of precursor protein p105 and p100. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor,IkB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS),pro-inflammatory cytokines (IL-1 & TNF,etc.),and viral infection,IkB is phosphorylated and degraded by proteosome. The active NFkB heterodimer is translocated into the nucleus and induces gene expression.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually.
免疫原
Synthetic peptide corresponding to the N-terminus of human NFkB p50