Cytochrome c (m.w. 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis,prior to caspase activation,DNA fragmentation,and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Mouse small intestine lysate can be used as a positive control. The cytochrome c antibody detects the 12 kDa cytochrome c from mouse and bovine samples.
免疫原
Bovine cytochrome c
来源宿主
兔
反应性
经检测验证,可与小鼠、牛种属来源的Cytochrome c Polyclonal蛋白发生免疫反应.
保存建议
提供的Cytochrome c Polyclonal抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Cytochrome c Polyclonal抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.