PCNA (Proliferating Cell Nuclear Antigen) is a 36 kDa protein also known as cyclin. In early S phase,PCNA has a very granular distribution and is absent from the nucleoli. At late S phase,PCNA is prominent in the nucleoli. There are two forms of PCNA protein present in cells,a soluble form sensitive to organic fixation and not involved in replication,and the other form is insoluble and is associated with ongoing DNA synthesis.
应用类型
Western blotting (0.5-4 ug/ml). Per researcher’s feedback, it can also be used in immunoprecipitation and Immunohistochemistry (frozen & paraffin). However, the optimal conditions should be determined individually.
免疫原
Synthetic peptide corresponding to the C-terminus of human PCNA