Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT,a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation.
应用类型
Western blotting (0.5-4 ug/ml). Based on researchers feed back, it also works in Immunohistochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually.
免疫原
Synthetic peptide surrounding amino acid 727 of human Oct-1
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Oct-1蛋白发生免疫反应.
保存建议
提供的Oct-1抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Oct-1抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.