p53DINP1 (p53-dependent damage-inducible nuclear protein 1) encodes two proteins termed p53DINP1- (27 kDa) and p53DINP1-B (18 kDa). p53DINP1 may regulate p53-dependent apoptosis through phosphorylation at Ser46 and induction of p53AIP1. p53DINP1 is expressed in many tissues and induced by a variety of stress agents including UV stress,mutagenic stress,heat shock,and oxidative stress.
应用类型
Western blotting (0.5-4 ug/ml) and Immunohistochemistry (5-10 ug/ml). However, the optimal dilution conditions should be determined individually. Human Lung tissue lysate can be used as a positive control. The antibody detects the 27 kDa p53DINP1- in human, mouse, and rat samples. A lower band (~18 kDa) which represents the p53DINP1-B was also detected in certain tissue and cells.
免疫原
Synthetic peptide mapping to the N-terminus of human p53DINP1
来源宿主
兔
反应性
经检测验证,可与人小鼠、大鼠种属来源的p53NIDP1蛋白发生免疫反应.
保存建议
提供的p53NIDP1抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该p53NIDP1抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.