PUMA (p53 upregulated modulator of apoptosis) is one of the pro-apoptotic Bcl-2 family members which are also transcriptional targets of p53. PUMA gene encodes two BH3 domain-containing proteins termed PUMA- and PUMA-B. PUMA proteins bind Bcl-2,localize to the mitochondria,and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
应用类型
Western blotting (0.5-4 ug/ml) and immunofluorescence (2-4 ug/ml). However, the optimal dilution conditions should be determined individually.
免疫原
Synthetic peptide mapping to the C-terminus of human PUMA.