Livin is a novel member in the IAP (inhibitor of apoptosis) protein family. Livin contains a single baculoviral IAP repeat (BIR) domain and a RING finger domain and has two isoforms termed Livin- and Livin-B. Transfection of Livin protected cells from apoptosis induced by FADD,BAX,RIP,RIP3 and DR6. Livin directly reacts with several caspases including Caspase-3,-7,and –9,and it inhibits the activation of caspase-9 induced by Apaf-1,cytochrome c and ATP.
应用类型
Western blotting (2-6 ug/ml). However, the optimal dilution conditions should be determined individually. The antibody detects a band at ~33 kDa and a weaker band at 30 kDa, corresponding to Livin and B. Raji cell lysate can be used as a positive control.
免疫原
Synthetic peptide surrounding amino acid near the C-terminus of the long form of human Livin
来源宿主
兔
反应性
经检测验证,可与人种属来源的Livin蛋白发生免疫反应.
保存建议
提供的Livin抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Livin抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.