Bmf (for Bcl-2-modifying factor) is a novel BH3-only protein recently identified in human and mouse. The BH3 domain in Bmf is required for both binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells,Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell’s actin cytoskeleton. Disruption of the actin cytoskeleton,either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix,triggers release and activation of Bmf,initiating the downstream apoptosis program.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~28 kDa BMF in sample from human, mouse and rat origins. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 9 of human BMF
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的BMF发生免疫反应.
保存建议
提供的BMF产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该BMF产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.