Fas (APO-1,CD95) is a 45 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or Fas ligand (FasL). FasL is a tumor necrosis factor (TNF)-related type II membrane protein and is expressed in various tissue and cells. Both Fas and FasL are thought to play an important role in the apoptosis processes that takes place during T cell development.
应用类型
Western blotting (1-4 ug/ml), immunoprecipitation, and Immunohistochemistry. However, the optimal conditions should be determined individually. HeLa cell lysate can be used as a positive control and an approximately 40 kDa band should be detected by Western blotting.