Cell death signal are transduced by death domain (DD),death effector domain (DED),and caspase recruitment domain (CARD) containing molecules. A novel protein that interacts with DED of caspase-8 and -10,and FADD was recently identified and designated DEDAF (for DED associated factor). DEDAF is identical to the transcriptional repressor RYBP. DEDAF interacts with FADD and augments the formation of CD95/FADD/caspase-8 complexes at the cytoplasm. DEDAF also interacts with DED-containing DNA biding protein (DEDD) in the nucleus,indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. A549 or HepG2 cell lysate can be used as a positive control. A 32 kDa band should be detected.
免疫原
Synthetic peptide surrounding amino acid 218 of human DEDAF
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的DEDAF蛋白发生免疫反应.
保存建议
提供的DEDAF抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该DEDAF抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.