The mammalian proto-oncogene Ras encodes 21 kDa proteins (p21Ras) which exists in either a physiologically quiescent GDP-binding state or a GTPbinding signal-emitting state. Interaction of p21Ras with GTPase activating protein (GAP) can increase hydrolysis of p21Ras-bound GTP by as much as 1000-fold. The product of the neurofibromatosis type 1 gene (NF1) has also been shown to exhibit p21Ras GAP activity.
应用类型
Western blotting (0.5-4 ug/ml) and in immunoprecipitation (10-20 ug/ml) and Immunohistochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate can be used as a positive control. The antibody recognizes ~120 kDa Ras GAP of human, mouse, rat, and bovine origins.
免疫原
Synthetic peptide surrounding amino acid 1025 of human RasGAP
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、牛种属来源的RasGAP蛋白发生免疫反应.
保存建议
提供的RasGAP抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该RasGAP抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.