Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage,a checkpoint kinase ATM phosphorylates and activates Chk2,which in turn phosphorylates and activates p53. Chk2 also phosphorylates and activates BRCA1,the product of a tumor suppressor gene that is mutated in breast and ovarian cancer.
应用类型
Western blotting (0.5-4 ug/ml) and immunofluorescence (5-10 ug/ml). However, the optimal conditions should be determined individually.
免疫原
Synthetic peptide corresponding to amino acid near the N-terminus of human Chk2
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Chk2蛋白发生免疫反应.
保存建议
提供的Chk2抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Chk2抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.