Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/caspase-9 pathway. Smac/DIABLO binds to IAPs (Inhibitor of Apoptosis Proteins) and neutralizes the inhibitory effect of IAPs. Analysis of the structural basis of Smac/DIABLO has revealed that the N-terminal amino acids are required for Smac/DIABLO to bind to IAPs and activation of caspases.
应用类型
Western blotting (0.5-4 ug/ml) and Immunohistochemistry (5-20 ug/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects the 25 kDa Smac/DIABLO in samples from human, mouse, and rat origins.
免疫原
Synthetic peptide surrounding amino acid 220 of mouse Smac/DIABLO
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Smac/DIABLO蛋白发生免疫反应.
保存建议
提供的Smac/DIABLO抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Smac/DIABLO抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.