Perforin (Pore-forming protein) is originally cloned from mouse cytotoxic T-lymphocyte (CTL). It is a 555-amino acid protein with a 21-amino acid signal peptide. Perforin is a cytolytic mediator produced by killer lymphocytes,and is stored in and released by cytoplasmic granules. Perforin is involved in the killing mediated by CTL which represents an important mechanism in the immune defense against tumors and virus infection.
应用类型
Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody detects both mouse and rat perforin. Cross activity to perforin of other species has not been determined.
免疫原
Recombinant rat perforin
来源宿主
兔
反应性
经检测验证,可与小鼠、大鼠种属来源的Perforin蛋白发生免疫反应.
保存建议
提供的Perforin抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 1% BSA, 0.02%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Perforin抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.