Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains two pathways,in which the key apoptotic proteases caspase-8 and caspase-9,respectively,are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is located to the ER,but not to the cytoplasm or mitochondrion. Caspase-12 is activated by ER stress,including disruption of ER calcium hemeostasis,and mediates ER stress-induced apoptosis. Caspase-12 is localized to the ER with several proteins that are involved in Alzheimer’s disease including gamma-secretase presenilin and B-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-B. Caspase-12 is ubiquitously expressed in mouse tissues.
应用类型
Western blotting (0.5-4 ug/ml). It detects procaspase-12 (55 kDa) and cleavage caspase-12 (36 kDa) on SDS-PAGE immunoblots. However, the optimal conditions should be determined individually.
免疫原
Synthetic peptide corresponding to residues surrounding amino acid 310 of mouse caspase-12
来源宿主
兔
反应性
经检测验证,可与小鼠、大鼠种属来源的Caspase-12蛋白发生免疫反应.
保存建议
提供的Caspase-12抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Caspase-12抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.