RIP (Receptor Interacting Protein) is a 74 kDa Ser/Thr kinase which interacts with CD95 (Fas/Apo1) receptor and the tumor necrosis factor receptor (TNFR1). It is a cell death domain adapter protein which can bind to the adapter proteins TRADD,RAID,and TRAF2. RIP contains an N-terminal region with homology to protein kinase,an intermediate domain capable of association with MAPKKK and a C-terminal region containing an intracellular death domain motif. RIP activates both p38 MAP kinase and SAPK families. In vitro,RIP induces apoptosis,as well as SAPK/JNK and NF-kB activation. RIP possesses kinase activity as it autophosphorylates itself on serine and threonine residues.
应用类型
Western blot analysis (2-6 ug/ml), immunoprecipitation and Immunohistochemistry. However, the optimal conditions should be determined individually.
免疫原
Synthetic peptide surrounding amino acid 645 of human RIP
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的RIP蛋白发生免疫反应.
保存建议
提供的RIP抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该RIP抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.