MSK1,a Mitogen and Stress activated protein Kinase,is activated by ERK as well as p38 MAPK in response to growth factors and various cellular stress stimuli. MSK1 resembles RSK by having two kinase domains connected by a regulator linker region. Phosphorylation of RSK1 at Ser 364,Ser381 and Thr574 is critical for RSK1 activity. These sites are analogous to Ser360,Ser376 and Thr581 of MSK1 and maybe important for MSK1 activity as well.
应用类型
Western blot (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Phospho-MSK1(Thr581) detects 90 kDa MSK1 only when phosphorylated at Thr581. The antibody does not cross-react with nonphosphorylated MSK1 or MSK1 phosphorylated at other sites. It does not cross-react with RSK1, RSK2 or RSK3.
免疫原
Synthetic peptide surrounding phospho-Thr581 of human MSK1
来源宿主
兔
反应性
经检测验证,可与人、小鼠种属来源的Phospho-MSK1蛋白发生免疫反应.
保存建议
提供的Phospho-MSK1抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 1% BSA, 0.02%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Phospho-MSK1抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.