Bid,a BH3 domain-containing proapoptotic Bcl-2 family member,is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity,which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In the animal model studies,Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
应用类型
Western blot analysis (0.5-5 ug/ml) also works well in immunoprecipitation (20 ug/ml) and Immunohistochemistry (20 ug/ml). However, the optimal conditions should be determined individually. The antibody detects the full length (22 kDa) mouse Bid. Reactivity to other species has not been tested.
免疫原
Synthetic peptide surrounding amino acid 45 of mouse Bid
来源宿主
兔
反应性
经检测验证,可与小鼠种属来源的Bid蛋白发生免疫反应.
保存建议
提供的Bid抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Bid抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.