The forkhead family of transcription factors is involved in tumorigenesis in rhabdomyosarcoma and acute leukemias. Within the family,three members (FKHR,AFX,AND FKHRL1) have sequence similarity to the nematode homolog Daf 16,recently shown to mediate signaling via a pathway involving IGFR1,P13-K and Akt. There are three potential Akt phosphorylation sites on the FKHR proteins. One site,FKHR Ser256 is found in the Forkhead domain and the other two are outside this domain. Recent studies show that phosphorylation of FKHR family members by Akt promotes cell survival. Phosphorylation regulates FKHR nuclear translocation and target gene transcription. Insulin also stimulates FKHR phosphorylation at Ser253.
应用类型
Western blotting (1-2 ug/ml). However, the optimal concentrations should be determined individually. Phospho-AFX (Ser193) antibody detects only the phosphorylated Ser193 of AFX and does not react with nonphosphorylated AFX.
免疫原
Synthetic peptide corresponding to residues surrounding Ser193 of human FoxO4/AFX
来源宿主
兔
反应性
经检测验证,可与人、小鼠种属来源的Phospho-FoxO4/AFX蛋白发生免疫反应.
保存建议
提供的Phospho-FoxO4/AFX抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 1% BSA和0.02% 叠氮钠的PBS缓冲液中.请将该Phospho-FoxO4/AFX抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.