Bad is a member of the Bcl-2 family protein that selectively dimerize with Bcl-xL and Bcl-2 resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser 136. Phosphorylation at these sites results in the binding of Bad to 14-3-3 proteins and the inhibition of Bad binding to Bcl-xL and Bcl-2. Recently,Akt has also been shown to promote cell survival via its ability to phosphorylate Bad at Ser136.
应用类型
Western blot analysis (1-2 ug/ml) and immunoprecipitation (5-20 ug/ml). However, the optimal conditions should be determined individually. The antibody detects 23 kDa Bad only when phosphorylated at Ser112.
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Ser112 of mouse Bad
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Phospho-Bad蛋白发生免疫反应.
保存建议
提供的Phospho-Bad抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有50%甘油, 1% BSA, 0.02%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Phospho-Bad抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.