Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control,DNA replication and chromatin organization. Lamin A is specifically cleaved by caspase-6 and therefore serves as a marker for caspase-6 activation. During apoptosis the 70 kDa lamin A is cleaved to a large (40-45 kDa) and small (23 kDa) fragment. The cleavage of lamins results in nuclear disregulation and cell death.
应用类型
Western blot analysis (0.5-4 ug/ml) and Immunohistochemistry (15-20 ug/ml). However, the optimal conditions should be determined individually. The antibody mainly detects full length (~70 kDa) and in a much lesser extent the cleaved fragments (45 kDa and 23 kDa) of Lamin A/C.
免疫原
Synthetic peptide surrounding amino acid 220 of human Lamin A