Fodrin is a universally expressed membrane-associated cytoskeletal protein consisting of - and B-subunits. The protein is important for maintaining normal membrane structure and supporting cell surface protein function. -Fodrin is one of the important targets cleaved by caspases during apoptosis. The full length 240 kDa protein can be cleaved at several sites within its sequence by activated caspases generating N-terminal 150 kDa,C-terminal 120 kDa,and 35 kDa major products. Cleavage of -Fodrin leads to membrane malfunction and cell shrinkage.
应用类型
Western blot analysis (0.5-4 ug/ml) and in in Immunohistochemistry (10-20 ug/ml).
免疫原
Synthetic peptide surrounding amino acid 162 of mouse alpha-Fodrin
来源宿主
兔
反应性
经检测验证,可与人种属来源的alpha-Fodrin蛋白发生免疫反应.
保存建议
提供的alpha-Fodrin抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该alpha-Fodrin抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.