Caspase-8,a member of the caspase-family of proteases,plays a key role in mediating Fas (CD95) and TNF induced apoptosis. Caspase-8 is synthesized as inactive pro-enzyme and activation of the enzyme involves proteolytic cleavage that leads to the release of the active p18 and p10 subunits. Activated caspase-8 is able to cleave and activate downstream caspases,such as caspase-3,-6,-7 and a death agonist member of the Bcl-2/Bcl-xL family,Bid,leading to apoptosis.
应用类型
Western blotting (0.5-4 ug/ml) in samples from mouse and rat origins. However, the optimal conditions should be determined individually. The antibody detects the 25 kDa large subunit of caspase-8. It does not detect the full-length of caspase-8.
免疫原
Synthetic peptide corresponding to residues surrounding amino acid 392 of mouse caspase-8
来源宿主
兔
反应性
经检测验证,可与小鼠、大鼠种属来源的Caspase-8 (Active)蛋白发生免疫反应.
保存建议
提供的Caspase-8 (Active)抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该Caspase-8 (Active)抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.