Akt,also referred to as PKB and RAC,is the cellular homologue of the viral oncogene v-Akt. This protein kinase is activated by insulin and various growth factors and functions in a pathway involving PI3 kinase as activation is sensitive to wortmanin. Recent evidence suggests that Akt functions to promote cell survival by actively inhibiting apoptosis,although the pathways involved have not been identified. Akt is activated by phospholipid binding and phosphorylation within the activation loop at Thr308 and within the C-terminus at Ser473.
应用类型
Western blotting (0.5-4 ug/ml) and immunoprecipitation (10-20 ug/ml). However, the optimal conditions should be determined individually. The phospho-Akt (Ser473) antibody detects Akt1 when phosphorylated at Ser473 and Akt2 and Akt3 when phosphorylated at equivalent sites.
免疫原
Synthetic phospho-peptide corresponding to residues surrounding Ser473 of mouse Akt
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Phospho-Akt蛋白发生免疫反应.
保存建议
提供的Phospho-Akt抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 1% BSA, 0.02%硫柳汞的PBS,缓冲液中.请将该Phospho-Akt抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.