IkB-alpha protein is a NF-kB inhibitory protein. It binds NF-kB and retains the complex in the cytoplasm，thereby preventing it from entering into nucleus and functioning as a transcription factor. Upon receiving a variety of signals，IkB-alpha undergoes phosphorylation at serine residues by an ubiquitin-dependent protein kinases and finally degraded by the proteasome. Free NF-kB then binds DNA and affects gene expression.
应用类型
Western blot （0.5-4 ug/ml）. However， the optimal conditions should be determined individually. Cross-reactivity to IkB-alpha of other species has not been determined.
免疫原
E. coli expressed mouse IkBa
来源宿主
兔
反应性
经检测验证，可与小鼠种属来源的IkBa蛋白发生免疫反应.
保存建议
提供的IkBa抗体产品为100 ug 无色溶液，其浓度为0.2 mg/ml，溶解于包含有50%甘油， 1% BSA和0.02%硫柳汞的PBS （pH 7.2）缓冲液中.请将该IkBa抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
