TLR4 (Toll-like receptor 4) is a mammalain homologue of the Drosophila toll protein. The human TLR4 is a type-1 transmembrane protein containing 799 amino acid residues. The extracellular domain has leucine-rich repeats and the intracellular domain is similar to that of IL-1 receptor. TLR4 is a receptor for lipopolysaccharide (LPS). In mice that are genetically tolerant to endotoxin,mutations have been identified in the intracellular domain of TLR4 that abolish LPS responsiveness. TLR4 uses a mechanism similar to that of the IL-1 receptor for signal transduction that leads to activation of NF-kB.
应用类型
Western blot analysis (0.5-4 ug/ml). The antibody is also suitable for flow cytometric analysis of cell surface expression of TLR4. However, the optimal conditions should be determined individually.
免疫原
Recombinant human TLR4
来源宿主
兔
反应性
经检测验证,可与人种属来源的TLR4蛋白发生免疫反应.
保存建议
提供的TLR4抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有50%甘油, 1% BSA, 0.02%硫柳汞的PBS (pH 7.2)缓冲液中.请将该TLR4抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.