G16 is the only heterotrimeric G protein with a restricted expression pattern in hematopoietic cells. Differentiation of promyelocytic cells leads to decreased expression of G16. The G protein is known to couple a large number of G protein-coupled receptors to PLC-B and can lead to production of the secondary messengers diacylglycerol and inositol phosphates. Targeted deletion of G15,a mouse orthologue of human G16,causes reduced C5 receptor signaling. G16 serves as a marker,in addition to CD34,for hematopoietic progenitor cells.
应用类型
Western blotting (0.5-4 ug/ml) and in Immunohistochemistry (5-20 ug/ml). However, the optimal conditions should be determined individually. The antibody detects G15 in samples from human, mouse, rat, and rabbit origins. It also detects G16 from samples of human origin. Reactivity to other species has not been determined.
免疫原
Synthetic peptide surrounding amino acid 356of human G 15
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Ga15/16蛋白发生免疫反应.
保存建议
提供的Ga15/16抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Ga15/16抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.