Gi2 is a 40-41 kDa alpha-subunit of heterotrimeric protein that shares sequence homology with Gi3 and Gi1. All these G proteins can be ADP-ribosylated at the carboxy terminal Cys of the CAAX sequence，interfering with receptor coupling. Gi2 is expressed widely in tissues and cell lines. Activation of PLC-B by Gi-coupled receptors is the result of release of GBg subunits from Gi. Gi2 regulates potassium channel activation. Mutation at Arg179 has been identified in tumors. This and the Q205L mutant inhibit adenylyl cyclase.
应用类型
Western blot （at 0.5-4 ug/ml） and immunoprecipitation. However， the optimal conditions should be determined individually.
免疫原
Synthetic peptide surrounding amino acid 229 of human Gai2
来源宿主
兔
反应性
经检测验证，可与人种属来源的Gai2蛋白发生免疫反应.
保存建议
提供的Gai2抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该Gai2抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
