Human p21-GTPase-activated protein kinase 1 (PAK1) is a functional homolog of STE20 of Saccharomyces cerevisiae. PAK1 is a downstream effector of the Rho-family small GTPases Rac and Cdc42. A serine/threonine kinase,PAK1 induces the formation of filopodia and lamellipodia in Swiss 3T3 cells. PAK1 inhibits myosin light chain kinase,and appears to play a role in the regulation of apoptosis by phosphorylating the death agonist Bad. Membrane targeting of PAK1 induces neurite outgrowth. PAK1 may also regulate NFkB activation.
应用类型
Western blotting (0.5-4 ug/ml) and immunoprecipitation (10-20 ug/ml). However, the optimal conditions should be determined individually.
免疫原
Recombinant human PAK1
来源宿主
兔
反应性
经检测验证,可与人种属来源的PAK1蛋白发生免疫反应.
保存建议
提供的PAK1抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有50%甘油, 1% BSA, 0.02%硫柳汞的PBS (pH 7.2)缓冲液中.请将该PAK1抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.