Akt,also referred to as PKB and RAC,is the cellular homologue of the viral oncogene v-Akt. This protein kinase is activated by insulin and various growth factors and functions in a pathway involving PI3 kinase as activation is sensitive to wortmanin. Recent evidence suggests that Akt functions to promote cell survival by actively inhibiting apoptosis.
应用类型
Western blotting (0.5-4 ug/ml), in immunoprecipitation (10-40 ug/ml), and Immunohistochemistry (10-40 ug/ml). However, the optimal conditions should be determined individually. The antibody detects Akt1, Akt2 and Akt3.
免疫原
Synthetic peptide corresponding to residue surrounding amino acid 80 of human Akt
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、牛种属来源的Akt/PKB蛋白发生免疫反应.
保存建议
提供的Akt/PKB抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Akt/PKB抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.