MyD88 was identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment. Dominant-negative form of MyD88 attenuates IL-1R-mediated NF-kB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor,which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18. MyD88 is a general adaptor protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Jurkat cell lysate can be used as a positive control and a 35 kDa band can be detected.
免疫原
Synthetic peptide corresponding to residues surrounding amino acids 282 of human MyD88
来源宿主
兔
反应性
经检测验证,可与人、小鼠种属来源的MYD88蛋白发生免疫反应.
保存建议
提供的MYD88抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该MYD88抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.