Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (Microtubule-associated Protein1 Light Chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form,LC3-I is processed and recruited to autophagosomes,where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.
应用类型
Western blot, IF
免疫原
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
来源宿主
兔
反应性
经检测验证,可与人种属来源的LC3蛋白发生免疫反应.
保存建议
提供的LC3 (APG8A) (Isoform A specific)抗体产品为无色液体.建议使用冰袋运输,收到产品后,请-20 °C储存.该产品有效期为12个月.