Tumor necrosis factor- (TNF-) is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF- is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-. TNF- converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bond metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins,including TNF receptor,TGF-,L-selectin adhesion molecule,and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and murine tissues.
应用类型
Western blotting (1-2 ug/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects 80-130 kDa bands, which may represent mature protein, precursor and glycosylated TACE. HeLa and Jurkat cell lysate can be used as positive control.
免疫原
Synthetic peptide surrounding amino acid 810 of human TACE
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的TACE蛋白发生免疫反应.
保存建议
提供的TACE抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 1% BSA, 0.02%硫柳汞的PBS (pH 7.2)缓冲液中.请将该TACE抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.