AIF (apoptosis inducing factor) was recently identified that causes chromatin condensation and DNA fragmentation. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation,which are the hallmarks of apoptosis. AIF is highly conserved between human and mouse and widely expressed.
应用类型
Western blotting (0.5-4 ug/ml). A ~67 kDa band should be detected. Jurkat cell lysate can be used as a positive control. However, the optimal conditions for your testing samples should be determined individually.
免疫原
Synthetic peptide surrounding amino acid 520 of human AIF
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的AIF蛋白发生免疫反应.
保存建议
提供的AIF抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该AIF抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.