TrkA,the high affinity Nerve Growth Factor (NGF) receptor,autophosphorylates on tyrosine to activate multiple effectors. Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain,and phosphorylation at these sites reflects Trk kinase activity.
应用类型
Western blotting (0.5-4 ug/ml) in immunoprecipitation, and Immunohistochemistry. However, the optimal conditions should be determined individually. The antibody detects ~140 kDa TrkA. It does not cross-react with TrkB or TrkC.
免疫原
Synthetic peptide surrounding amino acid 494 of human TrkA