NIK （NF-kB-inducing kinase） is a member of the MAP kinase kinase kinase family that binds TRAF2 and stimulates NF-kB activity. NIK was initially isolated from a human B cell cDNA library and contains 795 amino acids with an apparent molecular weight of slightly more than 97 kDa on SDS gel. NIK is a serine/threonine kinase and its kinase activity contributes to IkB phosphorylation. The carboxyl terminal segment of NIK binds TRAF2. A mutant NIK with intact carboxyl terminus but without the two lysine residues at its catalytic domain serves as a dominant-negative inhibitor for NF-kB activation. NIK also interacts with TRAF6 and mediates IL-1-induced NF-kB activation.
应用类型
Western blotting （0.5-4 ug/ml）. However， the optimal conditions should be determined individually
免疫原
Synthetic peptide surrounding amino acid 927of human NIK
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠、狗、仓鼠种属来源的NIK蛋白发生免疫反应.
保存建议
提供的NIK抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS （pH 7.2）缓冲液中.请将该NIK抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
