Cathepsin L is a normal lysosomal cysteine endoproteases that is expressed in all cells. The latent 39 kDa pro-Cathepsin L must be processed by cleavage of the 96 amino acid pro-peptide,to generate the active two-chain form made up of 21 and 5 kDa subunits. Mature Cathepsin L is normally localized in the lysosomes where it plays a major role in intracellular protein catabolism. The fact that Cathepsin L is also capable of degrading extracellular matrix proteins suggests that it may play a role in invasion and metastasis and could be a useful prognostic indicator for cancer.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Jurkat cell lysate (Cat.# 2401-100) can be used as a positive control. Detects both the proenzyme and the mature form of cathepsin L
免疫原
Synthetic peptide surrounding amino acid 223 of human Cathepsin L
来源宿主
兔
反应性
经检测验证,可与人种属来源的Cathepsin L 蛋白发生免疫反应.
保存建议
提供的Cathepsin L 抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.1% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Cathepsin L 抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.