Calpains are cytosolic,calcium-dependent,cysteine proteases that are ubiquitously expressed in mammalian and avian tissues. Type I calpain requires micromolar amounts of calcium,while type II calpain requires millimolar amounts of calcium.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The anti-calpain I antibody recognizes the large subunit (80 kDa) of human Calpain I. It does not recognize the smaller subunit of human Calpain I.
免疫原
Synthetic peptide surrounding amino acid 700 of rat Calpain I
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、牛、猪、兔种属来源的Calpain I蛋白发生免疫反应.
保存建议
提供的Calpain I抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS缓冲液中.请将该Calpain I抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.