Human perforin (cytolysin) is believed to be one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis. Perforin expression is constitutive on NK cells,but increases in resting XD8+ cytotoxic cells upon activation.
应用类型
Immunoprecipitation (2-8 ug/ml), Flow cytometry (2-8 ug/ml), Immunohistochemistry (2-8 ug/ml for both frozen and paraffin sections), Immunocytochemistry (2-8 ug/ml),. However, the optimal conditions should be determined individually. Recognizes the 70 kDa perforin in human and primate.
免疫原
Purified granules from human YT lymphoma cells
来源宿主
小鼠
反应性
经检测验证,可与人种属来源的Perforin蛋白发生免疫反应.
保存建议
提供的Perforin抗体产品为100 ug 无色溶液,其浓度为1.0 mg/ml,溶解于包含有1 mg/ml BSA和1.5 mM硫柳汞和50%甘油.的PBS缓冲液中.请将该Perforin抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.