IKK (IkB kinase- or IKK-1) is part of a large protein complex responsible for the inducible phosphorylation of IkB proteins. Human IKK- is an 85 kDa peptide that has been shown to activate NFkB by phosphorylation of IkB proteins. IKK interacts with its upstream kinase,NIK,and its downstream substrate,the IkB proteins. Mutations of IKK in its kinase domain lead to a dominant-negative phenotype that suppresses TNF- and IL-1B induced NFkB activation.
应用类型
Western blot (at 0.5-1 ug/ml) and immunoprecipitation (4-8 ug/ml). However, the optimal conditions should be determined individually.
免疫原
E. coli expressed recombinant human IKKa
来源宿主
兔
反应性
经检测验证,可与人种属来源的IKKa/IKK-1蛋白发生免疫反应.
保存建议
提供的IKKa/IKK-1抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 1% BSA, 0.02%硫柳汞的PBS (pH 7.2)缓冲液中.请将该IKKa/IKK-1抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.