Apoptosis can be inhibited by a group of proteins called inhibitors of apoptosis (IAPs). These proteins contain a BIR (baculovirus IAP repeat) domain near the amino-terminus. The BIR domain can bind some caspases. Many members of the IAP family of proteins block proteolytic activation of caspase-3 and –7. For example,XIAP,cIAP-1 and cIAP-2 appear to block cytochrome c-induced activation of caspase-9,thereby preventing initiation of the caspase cascade. Since cIAP-1 and cIAP-2 were first identified as components in the cytosolic death domain-induced complex associated with the TNF family of receptors,they may inhibit apoptosis by additional mechanisms.
应用类型
Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Mouse small intestine and rat kidney tissue lysate can be used as positive controls
免疫原
Synthetic peptide surrounding amino acid 530 of human cIAP-1
来源宿主
兔
反应性
经检测验证,可与小鼠、大鼠种属来源的cIAP-1蛋白发生免疫反应.
保存建议
提供的cIAP-1抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该cIAP-1抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.