PTP1B is the founding member of the protein tyrosine phosphatase (PTP) family,which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif,which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth,differentiation,mitotic cycle,and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase,as well as JAK2 and TYK2 kinases,which implicated the role of this PTP in cell growth control,and cell response to interferon stimulation.
应用类型
The antibody can be used for ELISA (0.25 ug/ml), Western blotting (0.5 – 2.5 ug/ml).
免疫原
Recombinant protein encoding amino acids 1-321 of human PTP1B
来源宿主
兔
反应性
经检测验证,可与人、小鼠种属来源的PTP1B蛋白发生免疫反应.
保存建议
提供的PTP1B抗体产品为100 ug 无色溶液,其浓度为0.25 mg/ml,溶解于包含有0.09% (W/V) 叠氮钠的PBS with 0.09% (W/V) sodium azide..请将该PTP1B抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.