Bid,a BH3 domain-containing proapoptotic Bcl-2 family member,is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity,which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl-2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In the animal model studies,Bid-deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
应用类型
Western blot analysis (0.5-4 ug/ml), immunoprecipitation (5-10 ug/ml), and Immunohistochemistry (20-40 ug/ml). However, the optimal conditions should be determined individually. The antibody detects 22 kDa human Bid
免疫原
Synthetic peptide surrounding amino acid 37 of human Bid
来源宿主
兔
反应性
经检测验证,可与人种属来源的Bid蛋白发生免疫反应.
保存建议
提供的Bid抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Bid抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.