AMPK2 alpha2 is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit,and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses,AMPK is activated,and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR),key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
应用类型
The antibody can be used for ELISA (0.25 ug/ml) and Western blotting (0.5 – 2.5 ug/ml).
免疫原
Synthetic peptide selected from the C-terminal region of human AMPK
来源宿主
兔
反应性
经检测验证,可与人、小鼠种属来源的AMPK⍺2蛋白发生免疫反应.
保存建议
提供的AMPK⍺2抗体产品为100 ug 无色溶液,其浓度为0.25 mg/ml,溶解于包含有0.09% (W/V) 叠氮钠的PBS缓冲液中.请将该AMPK⍺2抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.