Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS,Nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.
应用类型
The antibody can be used for ELISA (0.25 ug/ml), Western Blotting (2.5 – 5.0 ug/ml), Immunohistochemistry (2.5 – 5.0 ug/ml) and Immunofluorescence (5 - 25 ug/ml).
免疫原
Synthetic peptide selected from the N-terminal region of human Nestin
来源宿主
兔
反应性
经检测验证,可与人、小鼠种属来源的Nestin蛋白发生免疫反应.
保存建议
提供的Nestin抗体产品为100 ug 无色溶液,其浓度为0.25 mg/ml,溶解于包含有0.09% (W/V) 叠氮钠的PBS缓冲液中.请将该Nestin抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.