NANOG is a transcription regulator involved with inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extra-embryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes.
应用类型
The antibody can be used for ELISA (0.25 ug/ml) and Western Blotting (2.5 – 5.0 ug/ml).
免疫原
His fusion recombinant protein of human FOXP3
来源宿主
兔
反应性
经检测验证,可与人 种属来源的Nanog蛋白发生免疫反应.
保存建议
提供的Nanog抗体产品为100 ug 无色溶液,其浓度为0.25 mg/ml,溶解于包含有0.09% (W/V) 叠氮钠的PBS缓冲液中.请将该Nanog抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.