Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Similar to other caspases,caspase-6 is also synthesized as an inactive pro-enzyme that is processed in cells undergoing apoptosis. Together with caspase-3,caspase-6 is one of the major caspases in apoptotic cells,and functions downstream of apoptosis inhibitors Bcl-2 and Bcl-xL. Caspase-6 has also been shown involving in the proteolysis of poly (ADP-ribose) polymerase (PARP) and nuclear lamin A.
应用类型
Western blotting (0.5-4 ug/ml) and immunofluorescence. However, the optimal conditions should be determined individually. The antibody detects the large subunit (18 kDa) of the active caspase-6. The antibody does not recognize other caspases.
免疫原
Synthetic peptide surrounding amino acids mapping to the C-terminus of the large fragment of human caspase-6