Hexokinases phosphorylate glucose to produce glucose-6-phosphate,thus committing glucose to the glycolytic pathway. Alternative splicing of the gene for GCK results in three tissue-specific forms of glucokinase,one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase,this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in the gene have been associated with non-insulin dependent diabetes mellitus (NIDDM),also called maturity onset diabetes of the young,type 2 (MODY2); mutations have also been associated with persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
应用类型
The antibody can be used for ELISA (0.25 ug/ml), Western Blotting (0.5 – 2.5 ug/ml) and Immunohistochemistry (2.5 – 5.0 ug/ml).
免疫原
Synthetic peptide selected from the C-terminal region of human GCK
来源宿主
兔
反应性
经检测验证,可与人、小鼠种属来源的GCK (Glucokinase)蛋白发生免疫反应.
保存建议
提供的GCK (Glucokinase)抗体产品为100 ug 无色溶液,其浓度为0.25 mg/ml,溶解于包含有0.09% (W/V) 叠氮钠的PBS缓冲液中.请将该GCK (Glucokinase)抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.