In vertebrates there are four major glucose-phosphorylating isoenzymes,designated hexokinase I,II,III,and IV. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase activity is involved in the first step in several metabolic pathways. HK3 is bound to the outer mitochondrial membrane. Its hydrophobic N-terminal sequence may be involved in membrane bindng. It is the predominant hexokinase isozyme expressed in insuline-responsive tissues such as skeletal muscle. The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated wiht the N-terminus. Although found in NIDDM patients,genetic variations of HK2 do not contribute to the disease.
应用类型
The antibody can be used for ELISA (0.25 ug/ml), Western blotting (0.5 – 2.5 ug/ml) and Immunohistochemistry (2.5 – 5.0 ug/ml).
免疫原
Synthetic peptide selected from the N-terminal region of human HK2