PARP,a 116 kDa nuclear poly (ADP-ribose) polymerase,is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215,which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.
应用类型
Western blot analysis (0.5-4 ug/ml) and immunocytochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes only the large fragment of PARP (89 kDa) and does not react with the full length PARP. Camptothecin treated Jurkat cell lysate can be used as a positive control.
免疫原
Synthetic peptide correspond to the N-terminal residues of the catalytic domain of human PARP
来源宿主
兔
反应性
经检测验证,可与人种属来源的PARP (Cleaved)蛋白发生免疫反应.
保存建议
提供的PARP (Cleaved)抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有50%甘油, 1% BSA和0.02%硫柳汞的PBS缓冲液中.请将该PARP (Cleaved)抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.